Molecular Technology
Introduction
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Dr Graeme ClarkHead of Molecular Technology Service g.clark@crukscotlandinstitute.ac.uk |
The Molecular Technology Service offers a diverse range of services to individuals and research groups throughout the Institute. The main focus of the facility has centred around the continuing expansion of our Next Generation Sequencing (NGS) services, allied to which, we also offer a range of Single Cell services predominantly focussing on single cell RNAseq. We process samples for a variety of cancer associated projects, in both mouse and human derived materials. We offer a full end-to-end service, from initial study design & planning, through sample QC, full library preparation, sequencing and data return. Allied to these advanced techniques, we continue to offer a range of standard molecular tests covering, plasmid purifications, Sanger sequencing and mycoplasma screening.
The facility’s core aim is to provide an efficient and adaptive service to research groups and researchers within the Institute. We have gradually established a core offering centred around NGS, which covers bulk RNAseq, total RNAseq, ChIPseq, RRBS, amplicon-based and whole exome preparations, and are continually striving to add more assays and tests which can be readily utilised by groups across the Institute. We routinely undertake early preparatory meetings with researchers prior to proceeding with NGS based projects and conduct these alongside colleagues from the Bioinformatics & Computational Biology core group. These joint meetings enable us to thoroughly understand the research goals of specific projects and give appropriate advice in order to meet both the experimental and analytic goals of the project.
Example NextSeq 2000 primary data analysis, as viewed utilising Sequencing Analysis Viewer (SAV, Illumina). Represents key primary analysis data QC performed by MTS staff prior to full data analysis/data return.
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